Evaluation of sperm’s chromatin quality with acridine orange test, chromomycin A3 and aniline blue staining in couples with unexplained recurrent abortion
نویسندگان
چکیده
منابع مشابه
Evaluation of Protamine Level in Human Sperm Samples Using Chromomycin A3 and Aniline Blue Staining
Background: Current microscopic experimental methods cannot diagnose DNA damages present in spermatozoa .Therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. As reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. Also, chromomycin A3 is used for evaluation of the ...
متن کاملP-74: Cytochemical Evaluation of Sperm Chromatin and DNA Integrity in Couples with 3 Unexplained Recurrent Spontaneous Abortion
Background: The aim of this study was to examine the possible relationship between sperm DNA integrity and chromatin packaging evaluated by cytochemical assays, traditional sperm parameters and recurrent spontaneous abortion (RSA) of unknown origin. Materials and Methods: In this cohort study, 40 couples with a history of RSA and 40 couples with proven fertility were considered as case and cont...
متن کاملevaluation of protamine level in human sperm samples using chromomycin a3 and aniline blue staining
background: current microscopic experimental methods cannot diagnose dna damages present in spermatozoa .therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. as reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. also, chromomycin a3 is used for evaluation of the ...
متن کاملThe Effect of Vitamin E and Selenium on Sperm Chromatin Quality in Couples with Recurrent Miscarriage
Background and Aims: Due to the paucity of studies, association between the morphology and function of sperm and recurrent miscarriage (RM) is not yet completely known. Increased reactive oxygen species and decreased antioxidant levels in men have been shown to be associated with RM. Recently it has been accepted that antioxidant therapy can approve sperm parameters. The goal of this study was ...
متن کاملChromomycin A3 Staining Indicates Sperm Protamine Deficiency
Chromomycin A3 binds to DNA and Protamine -deficient spermatozoa look bright yellow,whereas normal spermatozoa appear dull yellow.
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ژورنال
عنوان ژورنال: Journal of Assisted Reproduction and Genetics
سال: 2009
ISSN: 1058-0468,1573-7330
DOI: 10.1007/s10815-009-9361-3